abstract：AIMS:This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional culture method. METHODS AND RESULTS:Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water sa...

journal_title：Journal of applied microbiology

authors： Bonetta S,Bonetta S,Ferretti E,Balocco F,Carraro E

更新日期：2010-05-01 00:00:00

abstract：AIMS:The presence of Brettanomyces bruxellensis is an important issue during winemaking because of its volatile phenols production capacities. The aim of this study is to provide information on the ability of residual B. bruxellensis populations to multiply and spoil finished wines during storage in bottles. METHODS A...

journal_title：Journal of applied microbiology

authors： Coulon J,Perello MC,Lonvaud-Funel A,de Revel G,Renouf V

更新日期：2010-04-01 00:00:00

abstract：AIMS:To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. METHODS AND RESULTS:We isolated strain MA2, showing high copper resistance up to the 1.5 mmol l(-1) concentration; it was also resistant to other metal...

journal_title：Journal of applied microbiology

authors： Osman O,Tanguichi H,Ikeda K,Park P,Tanabe-Hosoi S,Nagata S

更新日期：2010-04-01 00:00:00

abstract：AIMS:To identify proteins regulating antimicrobial peptide (AMP) resistance in Vibrio parahaemolyticus using membrane subproteome analysis. METHODS AND RESULTS:Three synthetic AMPs (Q4, Q6 and H1) and a natural one from fish (pleurocidin) were used for selection of AMP-resistant strains. Differential expression patter...

journal_title：Journal of applied microbiology

authors： Shen CJ,Kuo TY,Lin CC,Chow LP,Chen WJ

更新日期：2010-04-01 00:00:00

abstract：AIMS:To validate a real-time PCR test for the diagnosis of Glässer's disease, a major pig disease caused by Haemophilus parasuis. METHODS AND RESULTS:The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples o...

journal_title：Journal of applied microbiology

authors： Turni C,Pyke M,Blackall PJ

更新日期：2010-04-01 00:00:00

abstract：AIM:To develop a faster and easier in vivo method to screen compounds for anti-oxidant activity using a microbial system. METHODS AND RESULTS:Bacterial redox sensor-based assay systems were applied. The activities of SoxR and OxyR, the bacterial redox sensors, were monitored to probe the intracellular redox status thr...

journal_title：Journal of applied microbiology

authors： Park SJ,Chung HY,Lee JH

更新日期：2010-04-01 00:00:00

abstract：:The exploration of new source materials and the use of alternative isolation and identification methods have led to rapid expansion in the knowledge of diversity; in Lysobacter, 11 new species having been described since 2005, and in Stenotrophomonas with six new species since 2000. The new species of Lysobacter, isol...

journal_title：Journal of applied microbiology

authors： Hayward AC,Fegan N,Fegan M,Stirling GR

更新日期：2010-03-01 00:00:00

abstract：AIMS:To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS:Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in bi...

journal_title：Journal of applied microbiology

authors： Muñoz-Provencio D,Pérez-Martínez G,Monedero V

更新日期：2010-03-01 00:00:00

abstract：AIMS:This study aimed at characterizing the lactic acid bacteria microbiota and selecting mixed endogenous starters to be used for sourdough fermentation of spelt or emmer flours. METHODS AND RESULTS:Identification of lactic acid bacteria was carried out by partial sequencing of the 16S rRNA, recA, 16S/23S rRNA spacer...

journal_title：Journal of applied microbiology

authors： Coda R,Nionelli L,Rizzello CG,De Angelis M,Tossut P,Gobbetti M

更新日期：2010-03-01 00:00:00

abstract：AIMS:To evaluate the production and stability of laccases by Pleurotus ostreatus in liquid co-cultures with Trichoderma viride as a function of infection time and agitation rate. METHODS AND RESULTS:Pleurotus ostreatus cultures were infected with T. viride spores at 30 and 48 h. Maximal laccase volumetric activity was...

journal_title：Journal of applied microbiology

authors： Flores C,Casasanero R,Trejo-Hernández MR,Galindo E,Serrano-Carreón L

更新日期：2010-03-01 00:00:00

abstract：AIMS:Hypersensitivity pneumonitis of machinists associated with metalworking fluids (MWF) was recently linked to Mycobacterium immunogenum. In addition to Mycobacterium, impacts of continuous and massive contact to other micro-organisms, such as Pseudomonas, were little studied. This report intended to quantify and cha...

journal_title：Journal of applied microbiology

authors： Gilbert Y,Veillette M,Duchaine C

更新日期：2010-02-01 00:00:00

abstract：AIMS:To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS:Primer sets were designed after sequence analysis of...

journal_title：Journal of applied microbiology

authors： Baudoin E,Couillerot O,Spaepen S,Moënne-Loccoz Y,Nazaret S

更新日期：2010-01-01 00:00:00

abstract：AIMS:To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater...

journal_title：Journal of applied microbiology

authors： Altug G,Cardak M,Ciftci PS,Gurun S

更新日期：2010-01-01 00:00:00

abstract：AIMS:To validate the use of the air pouch system to predict and examine early immune responses induced by the presumptive probiotics Lactobacillus paracasei subsp. paracasei B112, DC205, DC215 and DC412 strains in the gut mucosa. METHODS AND RESULTS:Only the DC412 strain interacted strongly with the cells forming the ...

journal_title：Journal of applied microbiology

authors： Kourelis A,Zinonos I,Kakagianni M,Christidou A,Christoglou N,Yiannaki E,Testa T,Kotzamanidis C,Litopoulou-Tzanetaki E,Tzanetakis N,Yiangou M

更新日期：2010-01-01 00:00:00

abstract：AIM:To investigate the antimicrobial effect of ionization on bacteria in household refrigerators. METHODS AND RESULTS:Ionizer prototypes were tested with respect to their technical requirements and their ability to reduce surface and airborne contamination in household refrigerators. Ion and ozone production of the te...

journal_title：Journal of applied microbiology

authors： Kampmann Y,Klingshirn A,Kloft K,Kreyenschmidt J

更新日期：2009-12-01 00:00:00

abstract：AIMS:To identify and characterize adhesion-associated proteins in the potential probiotic Lactobacillus fermentum BCS87. METHODS AND RESULTS:Protein suspensions obtained from the treatment of Lact. fermentum BCS87 with 1 mol 1(-1) LiCl were analysed by Western blotting using HRP-labelled porcine mucus and mucin. Two a...

journal_title：Journal of applied microbiology

authors： Macías-Rodríguez ME,Zagorec M,Ascencio F,Vázquez-Juárez R,Rojas M

更新日期：2009-12-01 00:00:00

abstract：AIMS:To evaluate the performance of three Salmonella plating media (Rambach, Xylose Lysine Deoxycholate agar and modified Brilliant Green Agar plus Novobiocin) as part of the ISO 6579: 2002 (Annex D) on poultry environmental samples. METHODS AND RESULTS:The samples analysed were those for the European Union Salmonella...

journal_title：Journal of applied microbiology

authors： Carrique-Mas JJ,Barnes S,McLaren I,Davies R

更新日期：2009-12-01 00:00:00

abstract：AIMS:The absence of standardized methods for quantifying faecal indicator bacteria (FIB) in sand hinders comparison of results across studies. The purpose of the study was to compare methods for extraction of faecal bacteria from sands and recommend a standardized extraction technique. METHODS AND RESULTS:Twenty-two m...

journal_title：Journal of applied microbiology

authors： Boehm AB,Griffith J,McGee C,Edge TA,Solo-Gabriele HM,Whitman R,Cao Y,Getrich M,Jay JA,Ferguson D,Goodwin KD,Lee CM,Madison M,Weisberg SB

更新日期：2009-11-01 00:00:00

abstract：AIMS:To see the possibility of particle size distribution analyser (PSDA) in detecting concentration of lactobacillus contaminants in yeast fermentation. METHODS AND RESULTS:A PSDA was used to rapidly determine the size and concentration of lactobacillus and Saccharomyces cerevisiae. Data showed that the aerodynamic d...

journal_title：Journal of applied microbiology

authors： Kim H,Park K,Oh S,Chang IS

更新日期：2009-11-01 00:00:00

abstract：AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...

journal_title：Journal of applied microbiology

authors： Verma T,Garg SK,Ramteke PW

更新日期：2009-11-01 00:00:00

abstract：AIM:To develop a reliable and simple method to produce safe fertilizers from human excreta using urea for sanitation of faeces. METHODS AND RESULTS:Urea was added to faecal matter (17% dry matter) at concentrations of 0.5-2% (w/w) and inactivation of Salmonella enterica subspecies 1 serovar Typhimurium (Salm. Typhimur...

journal_title：Journal of applied microbiology

authors： Nordin A,Ottoson JR,Vinnerås B

更新日期：2009-11-01 00:00:00

abstract：AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...

journal_title：Journal of applied microbiology

authors： Wang L,Li Y,Mustapha A

更新日期：2009-11-01 00:00:00

abstract：AIMS:The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells. METHOD:Three different protocols (pre-, simultaneous and post-treatm...

journal_title：Journal of applied microbiology

authors： Búfalo MC,Figueiredo AS,de Sousa JP,Candeias JM,Bastos JK,Sforcin JM

更新日期：2009-11-01 00:00:00

abstract：AIM:To determine the structure of the chimeric chromosome X of bottom-fermenting yeasts. METHODS AND RESULTS:Eight cosmid clones carrying DNA from chromosome X of bottom-fermenting yeasts were selected by end-sequencing. Four of the cosmid clones had Saccharomyces cerevisiae (SC)-type and Saccharomyces bayanus (SB)-ty...

journal_title：Journal of applied microbiology

authors： Ogata T,Izumikawa M,Tadami H

更新日期：2009-10-01 00:00:00

abstract：AIMS:To investigate the changes in the surface properties of Lactobacillus rhamnosus GG during growth, and relate them with the ability of the Lactobacillus cells to adhere to Caco-2 cells. METHODS AND RESULTS:Lactobacillus rhamnosus GG was grown in complex medium, and cell samples taken at four time points and freeze...

journal_title：Journal of applied microbiology

authors： Deepika G,Green RJ,Frazier RA,Charalampopoulos D

更新日期：2009-10-01 00:00:00

abstract：AIMS:To determine the effects of water activity (a(W); 0.995-0.90), temperature (5, 18, 25 and 30 degrees C), time of incubation (7-35 days) and their interactions on tenuazonic acid (TA) production on 2% soybean-based agar by two Alternaria alternata strains isolated from soybean in Argentina. METHODS AND RESULTS:TA ...

journal_title：Journal of applied microbiology

authors： Oviedo MS,Ramirez ML,Barros GG,Chulze SN

更新日期：2009-10-01 00:00:00

abstract：AIMS:To study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria. METHODS AND RESULTS:Seed of rice cultivar PSBRc14 was collected from farmers' fields of irrigated lowland in southern Luzon, Philippines. ...

journal_title：Journal of applied microbiology

authors： Cottyn B,Debode J,Regalado E,Mew TW,Swings J

更新日期：2009-09-01 00:00:00

abstract：AIMS:To investigate the in vitro antibacterial activity and antifungal mode of action of flocculosin, a cellobiose lipid produced by Pseudozyma flocculosa. METHODS AND RESULTS:When tested against clinical bacterial isolates, the compound was particularly active against Gram-positive bacteria and its effect was not mit...

journal_title：Journal of applied microbiology

authors： Mimee B,Pelletier R,Bélanger RR

更新日期：2009-09-01 00:00:00

abstract：AIMS:To obtain bacteria with PKS (polyketide synthase) genes and antimicrobial activity from sponges. METHODS AND RESULTS:Eighteen bacteria with KS (ketosynthase) genes were identified by polymerase chain reaction (PCR) screening of 98 isolates from South China Sea sponges, Stelletta tenuis, Halichondria rugosa, Dysid...

journal_title：Journal of applied microbiology

authors： Zhang W,Zhang F,Li Z,Miao X,Meng Q,Zhang X

更新日期：2009-08-01 00:00:00

abstract：AIMS:To investigate the effect of media composition and agroindustrial residues on bovicin HC5 production by Streptococcus bovis HC5. METHODS AND RESULTS:Batch cultures of S. bovis HC5 were grown in basal medium containing different carbon and nitrogen sources. The activity of cell-free and cell-associated bovicin HC5...

journal_title：Journal of applied microbiology

authors： De Carvalho AA,Mantovani HC,Paiva AD,De Melo MR

更新日期：2009-07-01 00:00:00

abstract：AIMS:Lactobacilli adhere to the intestinal epithelium and this intimate association likely promotes retention in the gastrointestinal tract and communication with the immune system. The aim of this study was to investigate whether or not the quorum-sensing signalling molecule, autoinducer (AI)-2, was produced by Lactob...

journal_title：Journal of applied microbiology

authors： Buck BL,Azcarate-Peril MA,Klaenhammer TR

更新日期：2009-07-01 00:00:00

abstract：AIMS:To determine roles of cortex lytic enzymes (CLEs) in Bacillus megaterium spore germination. METHODS AND RESULTS:Genes for B. megaterium CLEs CwlJ and SleB were inactivated and effects of loss of one or both on germination were assessed. Loss of CwlJ or SleB did not prevent completion of germination with agents th...

journal_title：Journal of applied microbiology

authors： Setlow B,Peng L,Loshon CA,Li YQ,Christie G,Setlow P

更新日期：2009-07-01 00:00:00

abstract：AIMS:The present study focuses on the impact of two different drying technologies and the influence of protectants on process survival and storage stability of the two lactic acid bacterial strains Enterococcus faecium and Lactobacillus plantarum. METHODS AND RESULTS:After incubation with the protectants glucose, sucr...

journal_title：Journal of applied microbiology

authors： Strasser S,Neureiter M,Geppl M,Braun R,Danner H

更新日期：2009-07-01 00:00:00

abstract：AIMS:The aim of the study was to evaluate the in vitro antibacterial activity of glucosinolates and their enzymatic hydrolysis product against bacteria isolated from the human intestinal tract. METHODS AND RESULTS:Using a disc diffusion bioassay, different doses of intact glucosinolates and their corresponding hydroly...

journal_title：Journal of applied microbiology

authors： Aires A,Mota VR,Saavedra MJ,Rosa EA,Bennett RN

更新日期：2009-06-01 00:00:00

abstract：AIMS:To investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria. METHODS AND RESULTS:Culturable bacteria were isolated from buried tyre shreds and identified using fatty acid methyl ester analysis. Isolates belonged to taxonomic gr...

journal_title：Journal of applied microbiology

authors： Vukanti R,Crissman M,Leff LG,Leff AA

更新日期：2009-06-01 00:00:00

abstract：AIMS:To investigate the interactions of Salmonella enterica with abiotic and plant surfaces and their effect on the tolerance of the pathogen to various stressors. METHODS AND RESULTS:Salmonella strains were tested for their ability to form biofilm in various growth media using a polystyrene plate model. Strong biofil...

journal_title：Journal of applied microbiology

authors： Kroupitski Y,Pinto R,Brandl MT,Belausov E,Sela S

更新日期：2009-06-01 00:00:00

abstract：AIMS:To investigate the microbiota in marinated, vacuum-packed pork and to characterize isolated bacteria with regard to their spoilage potential. METHODS AND RESULTS:Laboratory marinated pork meat and commercial products from three Norwegian producers were examined. Lactic acid bacteria dominated in all products at t...

journal_title：Journal of applied microbiology

authors： Schirmer BC,Heir E,Langsrud S

更新日期：2009-06-01 00:00:00

abstract：AIMS:To study the behaviour of salmonellae inoculated in the dry, raw materials, related to chocolate confectionery manufacture, as affected by the strain, cell preparation method and storage temperature. METHODS AND RESULTS:Outbreak and non outbreak-associated salmonellae were used for the inoculation of cocoa butter...

journal_title：Journal of applied microbiology

authors： Komitopoulou E,Peñaloza W

更新日期：2009-06-01 00:00:00

abstract：:Dental chair units (DCUs) contain integrated systems that provide the instruments and services for a wide range of dental procedures. DCUs use water to cool and irrigate DCU-supplied instruments and tooth surfaces during dental treatment. Water is supplied to these instruments by a network of interconnected narrow-bor...

journal_title：Journal of applied microbiology

authors： Coleman DC,O'Donnell MJ,Shore AC,Russell RJ

更新日期：2009-05-01 00:00:00

abstract：AIMS:To monitor emissions of NH(3) and N(2)O during composting and link these to ammonia oxidation rates and the community structure of ammonia oxidizing bacteria (AOB). METHODS AND RESULTS:A laboratory-scale compost reactor treating organic household waste was run for 2 months. NH(3) emissions peaked when pH started ...

journal_title：Journal of applied microbiology

authors： Jarvis A,Sundberg C,Milenkovski S,Pell M,Smårs S,Lindgren PE,Hallin S

更新日期：2009-05-01 00:00:00